The Effects Of HIV Mutations On The Immune System Science C.J. Stimson Essay Example For Students

The Effects Of HIV Mutations On The Immune System Science C.J. Stimson Essay INTRODUCTIONThe topic of this paper is the human immunodeficiency virus, HIV, and whether or not mutations undergone by the virus allow it to survive in the immune system. The cost of treating all persons with AIDS in 1993 in the United States was $7.8 billion, and it is estimated that 20,000 new cases of AIDS are reported every 3 months to the CDC. This question dealing with how HIV survives in the immune system is of critical importance, not only in the search for a cure for the virus and its inevitable syndrome, AIDS (Acquired Immunodeficiency Syndrome), but also so that over 500,000 Americans already infected with the virus could be saved. This is possible because if we know that HIV survives through mutations then we might be able to come up with a type of drug to retard these mutations allowing the immune system time to expunge it before the onset of AIDS. BACKGROUNDIn order to be able to fully comprehend and analyze this question we must first ascertain what HIV is, how the body attempts to counter the effects of viruses in general, and how HIV infects the body. DefinitionHIV is the virus that causes AIDS. We will write a custom essay on The Effects Of HIV Mutations On The Immune System Science C.J. Stimson specifically for you for only $16.38 $13.9/page Order now HIV is classified as a RNA Retrovirus. A retrovirus uses RNA templates to produce DNA. For example, within the core of HIV is a double molecule of ribonucleic acid, RNA. When the virus invades a cell, this genetic material is replicated in the form of DNA . But, in order to do so, HIV must first be able to produce a particular enzyme that can construct a DNA molecule using an RNA template. This enzyme, called RNA-directed DNA polymerase, is also referred to as reverse transcriptase because it reverses the normal cellular process of transcription. The DNA molecules produced by reverse transcription are then inserted into the genetic material of the host cell, where they are co-replicated with the hosts chromosomes; they are thereby distributed to all daughter cells during subsequent cell divisions. Then in one or more of these daughter cells, the virus produces RNA copies of its genetic material. These new HIV clones become covered with protein coats and leave the cell to find other host cells where they can repeat the life cycle. The Body Fights BackAs viruses begin to invade the body, a few are consumed by macrophages, which seize their antigens and display them on their own surfaces. Among millions of helper T cells circulating in the bloodstream, a select few are programmed to ?read? that antigen. Binding the macrophage, the T cell becomes activated. Once activated, helper T cells begin to multiply. They then stimulate the multiplication of those few killer T cells and B cells that are sensitive to the invading viruses. As the number of B cells increases, helper T cells signal them to start producing antibodies. Meanwhile, some of the viruses have entered cells of the body the only place they are able to replicate. Killer T cells will sacrifice these cells by chemically puncturing their membranes, letting the contents spill out, thus disrupting the viral replication cycle. Antibodies then neutralize the viruses by binding directly to their surfaces, preventing them from attacking other cells. Additionally, they precipitate chemical reactions that actually destroy the infected cells. As the infection is contained, suppresser T cells halt the entire range of immune responses, preventing them from spiraling out of control. Memory T and B cells are left in the blood and lymphatic system, ready to move quickly should the same virus once again invade the body. HIVs Life CycleIn the initial stage of HIV infection, the virus colonizes helper T cells, specifically CD4+ cells, and macrophages, while replicating itself relatively unnoticed. As the amount of the virus soars, the number of helper cells falls; macrophages die as well. The infected T cells perish as thousands of new viral particles erupt from the cell membrane. Soon, though, cytotoxic T and B lymphocytes kill many virus-infected cells and viral particles. These effects limit viral growth and allow the body an opportunity to temporarily restore its supply of helper cells to almost normal concentrations. It is at this time the virus enters its second stage.Throughout this second phase the immune system functions well, and the net concentration of measurable virus remains relatively low. .uaa17ec01275f8cf402c7fd6b77b00f40 , .uaa17ec01275f8cf402c7fd6b77b00f40 .postImageUrl , .uaa17ec01275f8cf402c7fd6b77b00f40 .centered-text-area { min-height: 80px; position: relative; } .uaa17ec01275f8cf402c7fd6b77b00f40 , .uaa17ec01275f8cf402c7fd6b77b00f40:hover , .uaa17ec01275f8cf402c7fd6b77b00f40:visited , .uaa17ec01275f8cf402c7fd6b77b00f40:active { border:0!important; } .uaa17ec01275f8cf402c7fd6b77b00f40 .clearfix:after { content: ""; display: table; clear: both; } .uaa17ec01275f8cf402c7fd6b77b00f40 { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .uaa17ec01275f8cf402c7fd6b77b00f40:active , .uaa17ec01275f8cf402c7fd6b77b00f40:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .uaa17ec01275f8cf402c7fd6b77b00f40 .centered-text-area { width: 100%; position: relative ; } .uaa17ec01275f8cf402c7fd6b77b00f40 .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .uaa17ec01275f8cf402c7fd6b77b00f40 .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .uaa17ec01275f8cf402c7fd6b77b00f40 .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .uaa17ec01275f8cf402c7fd6b77b00f40:hover .ctaButton { background-color: #34495E!important; } .uaa17ec01275f8cf402c7fd6b77b00f40 .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .uaa17ec01275f8cf402c7fd6b77b00f40 .uaa17ec01275f8cf402c7fd6b77b00f40-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .uaa17ec01275f8cf402c7fd6b77b00f40:after { content: ""; display: block; clear: both; } READ: George Bush Essay Paper But after a period of time, the viral level rises gradually, in parallel with a decline in the helper population. These helper T and B lymphocytes are not lost because the bodys ability to produce new helper cells is impaired, but because the virus and cytotoxic cells are destroying them. This idea that HIV is not just evading the immune system but attacking and disabling it is what distinguishes HIV from other retroviruses.THE THEORIESThe hypothesis in question is whether or not the mutations undergone by HIV allow it to survive in the immune system. This idea was conceived by Martin A. Nowak, an immunologist at the University of Oxford, and his coworkers when they considered how HIV is able to avoid being detected by the immune system after it has infected CD4+ cells. The basis for this hypothesis was excogitated from the evolutionary theory and Nowaks own theory on HIV survival. Evolutionary TheoryThe evolutionary theory states that chance mutation in the genetic material of an individual organism sometimes yields a trait that gives the organism a survival advantage. That is, the affected individual is better able than its peers to overcome obstacles to survival and is also better able to reproduce prolifically. As time goes by, offspring that share the same trait become most abundant in the population, outcompeting other members until another individual acquires a more adaptive trait or until environmental conditions change in a way that favors different characteristics. The pressures exerted by the environment, then, determine which traits are selected for spread in a population. Nowaks Theory on HIV SurvivalWhen Nowak considered HIVs life cycle it seemed evident that the microbe was particularly well suited to evolve away from any pressures it confronted (this idea being derived from the evolutionary theory). For example, its genetic makeup changes constantly; a high mutation rate increases the probability that some genetic change will give rise to an advantageous trait. This great genetic variability stems from a property of the viral enzyme reverse transcriptase. As stated above, in a cell, HIV uses reverse transcriptase to copy its RNA genome into double-strand DNA. The virus mutates rapidly during this process because reverse transcriptase is rather error prone. It has been estimated that each time the enzyme copies RNA into DNA, the new DNA on average differs from that of the previous generation in one site. This pattern makes HIV one of the most variable viruses known.HIVs high replication rate further increases the odds that a mutation useful to the virus will arise. To fully appreciate the extent of HIV multiplication, look at the numbers published on it; a billion new viral particles are produced in an infected patient each day, and in the absence of immune activity, the viral population would on average double every two days. With the knowledge of HIVs great evolutionary potential in mind, Nowak and his colleagues conceived a scenario they thought could explain how the virus resists complete eradication and thus causes AIDS, usually after a long time span. Their proposal assumed that constant mutation in viral genes would lead to continuous production of viral variants able to evade the immune defenses operating at any given time. Those variants would emerge when genetic mutations led to changes in the structure of viral peptides recognized by the immune system. Frequently such changes exert no effect on immune activities, but sometimes they can cause a peptide to become invisible to the bodys defenses. The affected viral particles, bearing fewer recognizable peptides, would then become more difficult for the immune system to detect. The ModelUsing the theory that he had developed on the survival of HIV, along with the evolutionary theory, Nowak devised a model to simulate the dynamics and growth of the virus. The equations that formed the heart of the model reflected features that Nowak and his colleagues thought were important in the progression of HIV infection: the virus impairs immune function mainly by causing the death of CD4+ helper T cells, and higher levels of virus result in more T cell death. Also, the virus continuously produces escape mutants that avoid to some degree the current immunologic attack, and these mutants spread in the viral population. .ued1dd219f99f8d885e44bd354a99cb83 , .ued1dd219f99f8d885e44bd354a99cb83 .postImageUrl , .ued1dd219f99f8d885e44bd354a99cb83 .centered-text-area { min-height: 80px; position: relative; } .ued1dd219f99f8d885e44bd354a99cb83 , .ued1dd219f99f8d885e44bd354a99cb83:hover , .ued1dd219f99f8d885e44bd354a99cb83:visited , .ued1dd219f99f8d885e44bd354a99cb83:active { border:0!important; } .ued1dd219f99f8d885e44bd354a99cb83 .clearfix:after { content: ""; display: table; clear: both; } .ued1dd219f99f8d885e44bd354a99cb83 { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .ued1dd219f99f8d885e44bd354a99cb83:active , .ued1dd219f99f8d885e44bd354a99cb83:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .ued1dd219f99f8d885e44bd354a99cb83 .centered-text-area { width: 100%; position: relative ; } .ued1dd219f99f8d885e44bd354a99cb83 .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .ued1dd219f99f8d885e44bd354a99cb83 .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .ued1dd219f99f8d885e44bd354a99cb83 .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .ued1dd219f99f8d885e44bd354a99cb83:hover .ctaButton { background-color: #34495E!important; } .ued1dd219f99f8d885e44bd354a99cb83 .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .ued1dd219f99f8d885e44bd354a99cb83 .ued1dd219f99f8d885e44bd354a99cb83-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .ued1dd219f99f8d885e44bd354a99cb83:after { content: ""; display: block; clear: both; } READ: Kaiser William II - Cause of World War I Essay After awhile, the immune system finds the mutants efficiently, causing their population to shrink.The simulation managed to reproduce the typically long delay between infection by HIV and the eventual sharp rise in viral levels in the body. It also provided an explanation for why the cycle of escape and repression does not go on indefinitely but culminates in uncontrolled viral replication, the almost complete loss of the helper T cell population and the onset of AIDS.After the immune system becomes more active, survival becomes more complicated for HIV. It is no longer enough to replicate freely; the virus also has to be able to ward off immune attacks. Now is when Nowak predicts that selection pressure will produce increasing diversity in peptides recognized by immune forces. Once the defensive system has collapsed and is no longer an obstacle to viral survival, the pressure to diversify evaporates. In patients with AIDS, we would again anticipate selection for the fastest-growing variants and a decrease in viral diversity. Long-term studies involving a small number of patients have confirmed some of the modeling predictions. These investigations, conducted by several researchersincluding Andrew J. Leigh Brown of the University of Edinburgh, et al.tracked the evolution of the so-called V3 segment of a protein in the outer envelop of HIV for several years. V3 is a major target for antibodies and is highly variable. As the computer simulation predicted, viral samples obtained within a few weeks after patients become infected were alike in the V3 region. But during subsequent years, the region diversified, thus causing a rapid increase in the amount of V3 variants and a progressive decrease in the CD4+ cell count.The model presented by Nowak is extremely difficult to verify with clinical tests alone, largely because the diversified interactions between the virus and the immune system are impossible to monitor in detail. Consequently, Nowak turned to a computer simulation in which an initially homogeneous viral population evolved in response to immunologic pressure. He reasoned that if the mathematical model produced the known patterns of HIV progression, he could conclude the evolutionary scenario had some merit. To verify his model, he turned to the experiments done on the V3 protein segment in HIV. These experiments demonstrated that the peptides were mutating and that these mutations were leading to a decline in helper lymphocytes. CONCLUSIONBefore we begin to answer the question that this paper is investigating, an evaluation of our primary experiment source is necessary, this being a publication of Nowaks model. Upon evaluation of this source, a problem is exposed, this being that because there was no experiment performed to substantiate this model, we have no idea if the modeling predictions are true. Although there were previous non-directly related experiments ( i.e. , V3 experiment) that Nowak referred to to rationalize his model there was never an experiment done solely based on the model. Because the V3 findings were in accord with the findings of Nowaks model, we can assume that the model has some merit.This absence of an experiment is what leads to the boundaries that one encounters when experimenting with HIV mutations. These boundaries being that because HIV replicates and mutates non-linearly, it is impossible to chronicle all its viral dynamics scrupulously. The lack of experimental data based on Nowaks model along with the inadequacy of experiments dealing with HIV mutations leads to the conclusion that at present, there is no answer to this question. Although, other questions have been exposed, including: does the virus mutate at random or is it systematic? And how does the virus know where to mutate in order to continue surviving undetected?These are all questions that must first be answered before we even begin to try to determine if viral mutations are what allows HIV to survive in the immune system.